Rate of cluster buds induced by different gingkgo explants is not the same . among them , cluster buds can be obtained directly from stems and mostly from axillar bud , but the amount is little and differentiation rate is only 20 % 银杏不同外植体对丛生芽的诱导不同,银杏茎段培养可以直接得到丛生芽,但数量极少,而且大部分属于腋芽萌发,分化率20左右。
But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture 而茎段和胚诱导的愈伤组织基本上没有丛生芽发生,这可能和不同愈伤组织的内部结构有关。成熟胚的胚状体诱导率较低,诱导相当困难,在n6 bai
There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs , some callus can induce cluster buds , but others can not . differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing 但是不同器官诱导的愈伤组织对于丛生芽的诱导也是不同的,子叶诱导的愈伤组织的分化率较高,有36左右,并且继代次数的增加也能够增加分化的几率